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Loxo@Lilly Principal Scientist, Cell Line Generation in New York, New York

Loxo@Lilly conducts discovery research, clinical development and regulatory affairs for oncology, with the goal of creating a pipeline of therapies that have the potential to make a meaningful difference for patients. Loxo@Lilly was created in December 2019, combining the Lilly Research Laboratories oncology organization and Loxo Oncology, which was acquired by Lilly in early 2019. The unit has team members in Louisville, CO, Indianapolis, IN, New York City, NY, South San Francisco, CA, San Diego, CA, Stamford, CT, and Madrid, Spain.

We are looking for a highly capable and motivated laboratory-based scientist with strong experience in cell line generation and characterization. The Principal Scientist will play a key role in helping to establish and build out cell line generation/cell biology team that will be tasked with generating engineered cell lines for our preclinical programs in oncology. The primary responsibilities will include engineering cell lines for use in in vitro mechanistic assays and studies. Working in a collaborative environment, this individual, in addition to independently designing, executing, and optimizing experiments, will also provide supervision and mentorship to more junior team members. Additionally, the Principal Scientist will be tasked with analyzing data from experiments and presenting data to various cross functional team members and leadership as needed.

Roles and Responsibilities of the Position:

  • Construct knockout and expression vectors using mammalian plasmid and lentiviral, adenoviral, or retroviral systems. Viral titer measurement, and viral stock maintenance.

  • Generate engineered stable cell lines using transfection, CRISPR/Cas9 tools, lentiviral transduction, etc.

  • Optimize cell line development platforms to improve speed and reproducibility of cell line development.

  • Perform routine molecular and cell biology techniques like DNA/RNA/protein extraction, qPCR analysis, western blot analysis, fluorescent microscopy and so on.

  • Maintain cultures of frequently used suspension and adherent cell lines.

  • Deposit and archive cell lines in the company’s repository cell line bank.

  • Keep detailed and accurate laboratory records, present results at group meetings and project team meetings.

Required Qualifications and Preferred Background:

  • BA/BS with 8 years’ experience/MS with 6 years’ experience/PhD with 3+ years’ experience in biology, cell biology, molecular biology or a related field.

  • 8+ recent experience working with mammalian cell culture.

  • 1+ year of supervisory experience.

  • Tissue culture techniques such as cell propagation, cryo- preservation, and maintenance.

  • Proven cell culture experience with immortalized cell lines, cancer cell lines and/or primary cells.

  • Experience creating stable cell lines, transient transfections, viral transductions.

  • Experience with performing qPCR, ELISA, western blot, flow cytometry, and cell sorting, all in the context of mammalian cells. Fluorescent microscopy experience would be a plus.

  • Experience in RNAi, CRISPR, and chemical inhibition of cellular pathways.

  • Prior experience with various analytical reagents such as Calcein AM, Cell Titer Glo, Alamar Blue, Annexin, PI/7AAD, and immunofluorescent antibodies.

  • A high level of proficiency with many kinds of scientific software used to operate lab equipment and/or the ability to quickly master new software.

  • Experience and proficiency with data analysis using statistical software such as Excel, Prism, Spotfire or others.

  • Must be organized and efficient and able to work according to timelines.

  • Pharma/biotech drug discovery experience a plus.

  • Loxo Oncology, Inc. (Loxo) currently anticipates that the base salary for the Principal Scientist level position could range from between $103,200 to $151,423 and will depend, in part, on the successful candidate’s qualifications for the role, including education and experience. This position will also be eligible for a company bonus (depending, in part, on company and individual performance). In addition, Loxo provides a comprehensive benefit program to eligible employees, including vacation, medical, dental and vision benefits; participation in a 401(k) plan; life and accidental death and disability coverage; parental leave benefits and employee discounts for certain items. The compensation described above is subject to change and could be higher or lower than the range described.

Physical Demands/ Travel:

The physical demands of this job are consistent with laboratory bench setting and light office duties.

The physical demands here are representative of those that must be met by an employee to successfully perform the essential functions of this job.

Work Environment:

This position’s work environment is in a laboratory-based setting.

The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job.

*To perform this job successfully, an individual must be able to perform the role and responsibilities satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

Loxo Oncology, Inc. is an EEO/Affirmative Action Employer and does not discriminate on the basis of age, race, color, religion, gender, sexual orientation, gender identity, gender expression, national origin, protected veteran status, disability or any other legally protected status.

As a condition of employment with Eli Lilly and Company and its subsidiaries in the United States and Puerto Rico, you must be fully COVID-19 vaccinated and provide proof of vaccination satisfactory to the company (subject to applicable law).

Loxo Oncology, Inc. (“Loxo”) is committed to help individuals with disabilities participate in the workforce and ensure equal opportunity to compete for jobs. If you are an individual with a disability and require a reasonable accommodation to participate in the application process for positions at Loxo, please email loxoaccommodation@loxooncology.com for further assistance. Please note, this email address is intended for use to request an accommodation as a part of the application process. Any other correspondence will not receive a response.

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